The book summarizes the experience of Children’s Hospital of Fudan University in neonatal nursing assessment over the years. It covers key components of neonatal clinical nursing including assessment methodologies, assessment tools, and anticipatory assessment strategies. The assessment result can be referred to in diagnosis and treatment among newborns. A practical handbook, it can be will provide neonatal nurses with systematic guidance for nursing assessment, so that they can better develop individualized nursing plans for newborns and their families.